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10x chromium single cell immune profiling11/26/2023 ![]() Chromium Next GEM Single Cell V(D)J Reagent Kits v1.1 Chromium Single Cell V(D)J Reagent Kits v1.1 with Feature Barcoding technology for Cell Surface Protein Demonstrated Protocols. The additional information from antibody UMI (unique molecular identifier) counts enrich phenotype information of many canonical immune cell types compared to gene expression data alone, for example, naive and memory T cells, CD4+, and CD8+ T cells. Broad range of cell throughput per sample (100 - 10,000) Cell size flexibility, no lower limits User Guides. The plot ~5,000 shows peripheral blood mononuclear cells clustered by cell surface protein expression and visualized in Loupe Browser. Compatible assays: Chromium Single Cell Gene Expression, Chromium Single Cell Immune Profiling. Each method we tested showed advantages that could benefit immune cell profiling. Our products in action: Identify core immune cell subsets with easily conjugated 5’ Feature Barcode antibodiesĬore immune cell types are identified in the plot below with a 10-marker panel of antibodies conjugated with the 5’ Feature Barcode Antibody Conjugation Kit - Lightning-Link®. We benchmarked four commercially-available high-throughput single-cell systems: the Chromium (10x Genomics), the ddSEQ (Illumina and Bio-Rad), the scRNA-Seq System. Multiomic Cytometry 3’ – Single Cell Gene Expression solutionĥ’ Feature Barcode Antibody Conjugation Kit - Lightning-Link® - Oligos 1-10ĥ’ Feature Barcode Antibody Conjugation Kit - Lightning-Link® - Oligos 11-20ĥ’ Feature Barcode Antibody Conjugation Kit - Lightning-Link® - Oligos 21-30 Please update your browser or switch to Chrome, Firefox or Safari.Multiomic Cytometry 5’ – Single Cell Immune Profiling solution It is a wrapper around Illuminas bcl2fastq, with additional useful features that are specific to 10x libraries. box in the top left corner of the browser. Chromium Single Cell Immune Profiling provides a comprehensive approach to simultaneously examine cellular heterogeneity of the immune system, T- and B-cell repertoire diversity, and antigen specificity at single cell resolution. To identify the phenotype of the top T cell clonotypes, select Cell Types from the Split on Category. 14, 2023 /PRNewswire/ - 10x Genomics, Inc. 1 day ago &0183 &32 PLEASANTON, Calif., Sept. ![]() Generated data analyzed with 10x Genomics Cell Ranger and Loupe Browser can help to fully characterize the B-cells and T-cells involved in immunological responses. ![]() ![]() and the unique 10x barcodes are used to associate reads back to the. and cell surface proteins (CSP) at single-cell resolution using 10x Genomics Chromium Single Cell Immune Profiling and Illumina sequencing systems. It is done by partitioning thousands of cells into nanoliter-scale Gel. There are several ways to do this in Loupe Browser. The Chromium Single Cell Immune Profiling Solution provides a scalable microfluidic platform for simultaneous generation of full-length, paired V(D)J sequences, as well as gene expression, cell surface protein expression, and antigen specificity data at a single cell level. The Chromium Single Cell Immune Profiling Solution provides a scalable microfluidic platform for simultaneous generation of full-length, paired V (D)J sequences, as well as gene expression, cell surface protein expression, and antigen specificity data at a single cell level. Your browser is antiquated and no longer supported on this website. One key benefit of running an integrated 5' Gene Expression and V(D)J experiment is the ability to associate phenotypes with clonotypes of interest.
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